SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers to that protein
Primer Design | Department of Molecular and Cell Biology | University of Leicester
AB Vector - pAB-6xHis™
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Structural Biochemistry/Proteins/Purification/Affinity chromatography - Wikibooks, open books for an open world
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Polyhistidine-tag - wikidoc
SOLVED: Based on our vector we will need the Xhol and Ndel recognition sequence QQuestion 2 His:Tag Research the sequence for the Ndel cut site: Type your response here Xhol Ndel pET-28a
His-Tag | Definition & Data
His-Tag | Definition & Data
File:His-tag-primers.png - Wikimedia Commons
Polyhistidine-tag - Wikipedia
Harms Lab | Cloning of S100 with N-terminal TEV cleavage 6xHis-Tag
Addgene: pET28a His tag-FRB-Venus C
His-Tag | Definition & Data
Enzyme Purification by Affinity Column for Tagged Enzymes
biotechrabbit | RTS Wheat Germ LinTempGen Set, His6-tag - Vectors and Linear Template Kits - Rapid Translation Systems (RTS) - Cell-free Protein Synthesis - Products | leap and lead
pET-52 3C/LIC Vector Kit - Novagen | 71571
pET-28a - $162.00
When your his-tagged constructs don't bind—troubleshooting your protein purification woes
AB Vector - pAB-6xHis-MBP™
Success #11: 10xHis Tag Insertion by Site-Directed Mutagenesis | Civic Bioscience
SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end: Please add a restriction enzyme cut site to each of the original primers to that protein
