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Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays | BMC Research Notes | Full Text
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Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE) | Nature Communications
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Evaluation of RT-qPCR primer efficiencies. The amplification efficiency... | Download Scientific Diagram
![SOLVED: I need to calculate the primer concentration after PCR cycle. My DNA segment (798 bp) is amplified by PCR. The 50 uL mixture of PCR reaction. Primer concentration is 2 uM. SOLVED: I need to calculate the primer concentration after PCR cycle. My DNA segment (798 bp) is amplified by PCR. The 50 uL mixture of PCR reaction. Primer concentration is 2 uM.](https://cdn.numerade.com/ask_images/98e7f0d592204f928d39e0d8c9fe3086.jpg)