![SOLVED: QNo.3. Calculate Tm of the following primers Gene: Sequence Melting temperature Forward primer TCGAGTCGCGTCCACC Reverse primer GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR= Cytogenetic location= d. Targeted SOLVED: QNo.3. Calculate Tm of the following primers Gene: Sequence Melting temperature Forward primer TCGAGTCGCGTCCACC Reverse primer GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR= Cytogenetic location= d. Targeted](https://cdn.numerade.com/ask_images/479db41e130a4d759330f82f59f9f3f4.jpg)
SOLVED: QNo.3. Calculate Tm of the following primers Gene: Sequence Melting temperature Forward primer TCGAGTCGCGTCCACC Reverse primer GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR= Cytogenetic location= d. Targeted
![Table III from Primer design with specific PCR product size using Memetic algorithm | Semantic Scholar Table III from Primer design with specific PCR product size using Memetic algorithm | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/9070c4f4c7911992afca37e68edac89653eada11/6-TableIII-1.png)
Table III from Primer design with specific PCR product size using Memetic algorithm | Semantic Scholar
![Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE) | Nature Communications Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE) | Nature Communications](https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Fs41467-022-29500-4/MediaObjects/41467_2022_29500_Fig1_HTML.png)