![SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (% SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%](https://cdn.numerade.com/ask_previews/6f0c47ee-38d7-4778-b4d8-d117b7d1affe_large.jpg)
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
![Primer design strategy for denaturation bubble-mediated strand exchange amplification - ScienceDirect Primer design strategy for denaturation bubble-mediated strand exchange amplification - ScienceDirect](https://ars.els-cdn.com/content/image/1-s2.0-S0003269719312813-fx1.jpg)
Primer design strategy for denaturation bubble-mediated strand exchange amplification - ScienceDirect
![SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly On iength and composition of the primer(s) An annealing temperature 5"€ SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly On iength and composition of the primer(s) An annealing temperature 5"€](https://cdn.numerade.com/ask_images/4923a58bd9124b0c9cc690a3580c29e8.jpg)