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Optimization of allele-specific primer and blocker Tm. CT values are... | Download Scientific Diagram
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![How would one work out the Tm value for a single primer? What is the Tm value for this primer sequence? 5'-GCCAGTCAAACAGTCAGTAA-3' B. ~47oC C. ~50oC D. ~53oC E. ~56oC : r/a:t5_318cy How would one work out the Tm value for a single primer? What is the Tm value for this primer sequence? 5'-GCCAGTCAAACAGTCAGTAA-3' B. ~47oC C. ~50oC D. ~53oC E. ~56oC : r/a:t5_318cy](https://external-preview.redd.it/wF4MLnRL12_90I71Zx1wWdRIoI2s-vIYa8MQSETa85k.jpg?width=640&crop=smart&auto=webp&s=08fab9e34c1ad97a31d610b2709f6f30540100e0)