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How would one work out the Tm value for a single primer? What is the Tm value for this primer sequence? 5'-GCCAGTCAAACAGTCAGTAA-3' B. ~47oC C. ~50oC D. ~53oC E. ~56oC : r/a:t5_318cy
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Nucleic Acid Design Applications Polymerase Chain Reaction (PCR) Calculating Melting Temperature (Tm) PCR Primers Design. - ppt download
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